Phosphoribosyl pyrophosphate amidotransferase (PPAT) Mouse Monoclonal Antibody [Clone ID: OTI1B8]

SKU CF504769

Carrier-free (BSA/glycerol-free) PPAT mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)

Product Data

• Clone Name: OTI1B8
• Application: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivity: Dog, Human, Monkey, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 42-278 of human PPAT(NP_002694) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 57.2 kDa
• Gene Name: phosphoribosyl pyrophosphate amidotransferase
• Database Link:
  NP_002694
  Q06203
• Background: The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: ATASE; GPAT; PRAT

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism