Fetuin A (AHSG) Mouse Monoclonal Antibody [Clone ID: OTI2H2]

CAT#: CF504670

Carrier-free (BSA/glycerol-free) AHSG mouse monoclonal antibody, clone OTI2H2 (formerly 2H2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AHSG (Cat# RC208344, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AHSG(Cat# TA504670). Positive lysates LY400610 (100ug) and LC400610 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-AHSG monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AHSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504670)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-AHSG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504670)

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  HEK293T cells transfected with either RC208344 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AHSG antibody (TA504670), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2H2
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AHSG(NP_001613) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.2 kDa
• Gene Name: alpha 2-HS glycoprotein
• Database Link:
  NP_001613
  Entrez Gene 197 Human
  UniProt ID P02765
• Background: Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq]
• Synonyms: A2HS; AHS; FETUA; HSGA

Reference Data

• Protein Families: Druggable Genome, Secreted Protein