SAT2 Mouse Monoclonal Antibody [Clone ID: OTI1C9]

CAT#: CF504633

Carrier-free (BSA/glycerol-free) SAT2 mouse monoclonal antibody, clone OTI1C9 (formerly 1C9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SAT2 (Cat# RC204044, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAT2(Cat# TA504633). Positive lysates LY408821 (100ug) and LC408821 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504633)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504633)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504633)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504633)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504633)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504633)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-SAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504633)

• Anti-SAT2 mouse monoclonal antibody (TA504633) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAT2 (RC204044).

• HEK293T cells transfected with either RC204044 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SAT2 antibody (TA504633), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-SAT2 antibody (TA504633), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-SAT2 antibody (TA504633), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1C9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SAT2(NP_597998) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19 kDa
• Gene Name: spermidine/spermine N1-acetyltransferase family member 2
• Database Link:
  NP_597998
  Entrez Gene 112483 Human
  UniProt ID Q96F10
• Synonyms: SSAT2

Reference Data

• Protein Pathways: Arginine and proline metabolism, Metabolic pathways