Carboxypeptidase A2 (CPA2) Mouse Monoclonal Antibody [Clone ID: OTI1C7]

CAT#: CF504622

Carrier-free (BSA/glycerol-free) CPA2 mouse monoclonal antibody, clone OTI1C7 (formerly 1C7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CPA2 (Cat# RC202719, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CPA2(Cat# TA504622). Positive lysates LY419697 (100ug) and LC419697 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC202719 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CPA2 antibody (TA504622), and then analyzed by flow cytometry.

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  Anti-CPA2 mouse monoclonal antibody (TA504622) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CPA2 (RC202719).

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504622)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-CPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504622)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504622)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504622)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504622)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504622)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504622)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504622)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504622)

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Specifications

Product Data

• Clone Name: OTI1C7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CPA2(NP_001860) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.9 kDa
• Gene Name: Homo sapiens carboxypeptidase A2 (CPA2), mRNA.
• Database Link:
  NP_001860
  Entrez Gene 232680 MouseEntrez Gene 296959 RatEntrez Gene 1358 Human
  UniProt ID P48052
• Background: Three different forms of human pancreatic procarboxypeptidase A have been isolated. The encoded protein represents the A2 form, which is a monomeric protein with different biochemical properties from the A1 and A3 forms. The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues and is a secreted protein. [provided by RefSeq]
• Synonyms: carboxypeptidase A2 (pancreatic)

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein