GADD34 (PPP1R15A) Mouse Monoclonal Antibody [Clone ID: OTI2B11]

CAT#: CF504309

Carrier-free (BSA/glycerol-free) PPP1R15A mouse monoclonal antibody, clone OTI2B11 (formerly 2B11)

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPP1R15A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human GADD34 plasmid (RC200581, hGADD34), mouse GADD34 plasmid (MR227514, mGADD34) using anti-GADD34 antibody TA504309(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

• 

  Anti-PPP1R15A mouse monoclonal antibody (TA504309) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP1R15A (RC200581).

• 

  HEK293T cells transfected with either RC200581 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPP1R15A antibody (TA504309), and then analyzed by flow cytometry.

• 

  Flow cytometric Analysis of Hela cells, using anti-PPP1R15A antibody (TA504309), (Red), compared to a nonspecific negative control antibody, (Blue).

• 

  Flow cytometric Analysis of Jurkat cells, using anti-PPP1R15A antibody (TA504309), (Red), compared to a nonspecific negative control antibody, (Blue).

Show all 6 images

Specifications

Product Data

• Clone Name: OTI2B11
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPP1R15A(NP_055145) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.3 kDa
• Gene Name: protein phosphatase 1 regulatory subunit 15A
• Database Link:
  NP_055145
  Entrez Gene 17872 MouseEntrez Gene 23645 Human
  UniProt ID O75807
• Background: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008]
• Synonyms: GADD34

Reference Data

• Protein Families: Druggable Genome