Epsin 2 (EPN2) Mouse Monoclonal Antibody [Clone ID: OTI1C2]

CAT#: CF504297

Carrier-free (BSA/glycerol-free) EPN2 mouse monoclonal antibody, clone OTI1C2 (formerly 1C2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EPN2 (Cat# RC213652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPN2(Cat# TA504297).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPN2 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504297)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504297)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504297)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504297)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504297)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504297)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504297)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-EPN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504297)

• Anti-EPN2 mouse monoclonal antibody (TA504297) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EPN2 (RC213652).

• Flow cytometric Analysis of Hela cells, using anti-EPN2 antibody (TA504297), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-EPN2 antibody (TA504297), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1C2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EPN2(NP_055779) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.3 kDa
• Gene Name: Homo sapiens epsin 2 (EPN2), transcript variant 2, mRNA.
• Database Link:
  NP_055779
  Entrez Gene 13855 MouseEntrez Gene 60443 RatEntrez Gene 702600 MonkeyEntrez Gene 22905 Human
  UniProt ID O95208
• Background: This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
• Synonyms: EHB21

Reference Data

• Protein Pathways: Endocytosis