FMRP (FMR1) Mouse Monoclonal Antibody [Clone ID: OTI2C3]

CAT#: CF504293

Carrier-free (BSA/glycerol-free) FMR1 mouse monoclonal antibody, clone OTI2C3 (formerly 2C3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FMR1 (RC222699, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FMR1 (1:500).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FMR1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504293)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504293)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504293)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504293)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504293)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504293)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504293)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504293)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FMR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504293)

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Specifications

Product Data

• Clone Name: OTI2C3
• Applications: IHC, WB
• Recommended Dilution: WB 1:200~1000, IHC 1:150
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 26-279 of human FMR1(NP_002015) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71 kDa
• Gene Name: Homo sapiens FMRP translational regulator 1 (FMR1), transcript variant ISO1, mRNA.
• Database Link:
  NP_002015
  Entrez Gene 14265 MouseEntrez Gene 24948 RatEntrez Gene 481070 DogEntrez Gene 700138 MonkeyEntrez Gene 2332 Human
  UniProt ID Q06787
• Background: The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene. [provided by RefSeq, May 2010]
• Synonyms: FMRP; FRAXA; POF; POF1; POFX

Reference Data

• Protein Families: Druggable Genome