ALOX15 Mouse Monoclonal Antibody [Clone ID: OTI3G8]

CAT#: CF504250

Carrier-free (BSA/glycerol-free) ALOX15 mouse monoclonal antibody, clone OTI3G8 (formerly 3G8)

Product Images

Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human ALOX15 plasmid (RC206621, lane 2), mouse ALOX15 plasmid (MR226258, lane 3) using anti-ALOX15 antibody TA504250 (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

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  Immunohistochemical staining of paraffin-embedded human lung tissue within the normal limits using anti-ALOX15 mouse monoclonal antibody (TA504250). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 minutes (1:150).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ALOX15 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504250)

• Anti-ALOX15 mouse monoclonal antibody (TA504250) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALOX15 (RC206621).

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Specifications

Product Data

• Clone Name: OTI3G8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Rat, Monkey
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.6 kDa
• Gene Name: Homo sapiens arachidonate 15-lipoxygenase (ALOX15), mRNA.
• Database Link:
  NP_001131
  Entrez Gene 81639 RatEntrez Gene 709212 MonkeyEntrez Gene 246 Human
  UniProt ID P16050
• Synonyms: 12-LOX; 15-LOX-1; 15LOX-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Linoleic acid metabolism, Metabolic pathways