APMAP Mouse Monoclonal Antibody [Clone ID: OTI4F6]

CAT#: CF504220

Carrier-free (BSA/glycerol-free) C20orf3 mouse monoclonal antibody, clone OTI4F6 (formerly 4F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY C20orf3 (Cat# RC202410, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C20orf3(Cat# TA504220). Positive lysates LY402795 (100ug) and LC402795 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-C20orf3 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-C20orf3 monoclonal antibody (1:200).

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  Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-C20orf3 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504220)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504220)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504220)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504220)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504220)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504220)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504220)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504220)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504220)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504220)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-C20orf3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504220)

• Flow cytometric Analysis of Hela cells, using anti-C20orf3 antibody (TA504220), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-C20orf3 antibody (TA504220), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI4F6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C20orf3(NP_065392) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.3 kDa
• Gene Name: Homo sapiens adipocyte plasma membrane associated protein (APMAP), mRNA.
• Database Link:
  NP_065392
  Entrez Gene 71881 MouseEntrez Gene 366227 RatEntrez Gene 608089 DogEntrez Gene 704948 MonkeyEntrez Gene 57136 Human
  UniProt ID Q9HDC9
• Background: CHMP1B belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]). [supplied by OMIM]
• Synonyms: BSCv; C20orf3

Reference Data

• Protein Families: Transmembrane