NLN Mouse Monoclonal Antibody [Clone ID: OTI3A12]

CAT#: CF503978

Carrier-free (BSA/glycerol-free) NLN mouse monoclonal antibody, clone OTI3A12 (formerly 3A12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NLN (RC212447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NLN. Positive lysates LY412353 (100ug) and LC412353 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503978)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503978)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503978)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503978)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503978)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503978)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503978)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503978)

• HEK293T cells transfected with either RC212447 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NLN antibody (TA503978), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-NLN antibody (TA503978), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-NLN antibody (TA503978), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3A12
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NLN(NP_065777) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80.5 kDa
• Gene Name: neurolysin
• Database Link:
  NP_065777
  Entrez Gene 75805 MouseEntrez Gene 117041 RatEntrez Gene 57486 Human
  UniProt ID Q9BYT8
• Background: This gene encodes a member of the metallopeptidase M3 protein family that cleaves neurotensin at the Pro10-Tyr11 bond, leading to the formation of neurotensin(1-10) and neurotensin(11-13). The encoded protein is likely involved in the termination of the neurotensinergic signal in the central nervous system and in the gastrointestinal tract.
• Synonyms: AGTBP; EP24.16; MEP; MOP

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Renin-angiotensin system