Aminopeptidase A (ENPEP) Mouse Monoclonal Antibody [Clone ID: OTI3E7]

CAT#: CF503962

Carrier-free (BSA/glycerol-free) ENPEP mouse monoclonal antibody, clone OTI3E7 (formerly 3E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENPEP (RC210521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ENPEP. Positive lysates LY419610 (100ug) and LC419610 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ENPEP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503962)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503962)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503962)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503962)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503962)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503962)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503962)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503962)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503962)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ENPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503962)

• Anti-ENPEP mouse monoclonal antibody (TA503962) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ENPEP (RC210521).

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Specifications

Product Data

• Clone Name: OTI3E7
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human roecombinant protein of human ENPEP(NP_001968) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 109.1 kDa
• Gene Name: Homo sapiens glutamyl aminopeptidase (ENPEP), transcript variant 1, mRNA.
• Database Link:
  NP_001968
  Entrez Gene 2028 Human
  UniProt ID Q07075
• Synonyms: APA; CD249; gp160

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Transmembrane
• Protein Pathways: Renin-angiotensin system