DPP9 Mouse Monoclonal Antibody [Clone ID: OTI2E3]

CAT#: CF503960

Carrier-free (BSA/glycerol-free) DPP9 mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DPP9 (Cat# RC229388, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP9(Cat# TA503960). Positive lysates LY403380 (100ug) and LC403380 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (50ug per lane) from 9 cell lines lysates by using anti-DPP9 monoclonal antibody(TA503960, 1:500)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DPP9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503960)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DPP9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503960)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DPP9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503960)

• 

  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DPP9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503960)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DPP9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503960)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DPP9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503960)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DPP9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503960)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DPP9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503960)

• Flow cytometric Analysis of Jurkat cells, using anti-DPP9 antibody (TA503960), (Red), compared to a nonspecific negative control antibody, (Blue).

Show all 11 images

Specifications

Product Data

• Clone Name: OTI2E3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DPP9(NP_631898) produecd in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.4 kDa
• Gene Name: dipeptidyl peptidase 9
• Database Link:
  NP_631898
  Entrez Gene 224897 MouseEntrez Gene 301130 RatEntrez Gene 91039 Human
  UniProt ID Q86TI2
• Background: This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized. [provided by RefSeq]
• Synonyms: DP9; DPLP9; DPRP-2; DPRP2

Reference Data

• Protein Families: Druggable Genome, Protease