DUSP23 Mouse Monoclonal Antibody [Clone ID: OTI3C10]

CAT#: CF503944

Carrier-free (BSA/glycerol-free) DUSP23 mouse monoclonal antibody, clone OTI3C10 (formerly 3C10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DUSP23 (Cat# RC200171, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DUSP23(Cat# TA503944). Positive lysates LY402621 (100ug) and LC402621 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DUSP23 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-DUSP23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503944)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DUSP23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503944)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DUSP23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503944)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DUSP23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503944)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DUSP23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503944)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DUSP23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503944)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DUSP23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503944)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DUSP23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503944)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DUSP23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503944)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DUSP23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503944)

• Anti-DUSP23 mouse monoclonal antibody (TA503944) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DUSP23 (RC200171).

• Flow cytometric Analysis of Hela cells, using anti-DUSP23 antibody (TA503944), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-DUSP23 antibody (TA503944), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3C10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DUSP23(NP_060293) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.4 kDa
• Gene Name: Homo sapiens dual specificity phosphatase 23 (DUSP23), transcript variant 3, mRNA.
• Database Link:
  NP_060293
  Entrez Gene 54935 Human
  UniProt ID Q9BVJ7
• Synonyms: DUSP25; LDP-3; MOSP; VHZ

Reference Data

• Protein Families: Druggable Genome, Phosphatase