TUBAL3 Mouse Monoclonal Antibody [Clone ID: OTI1G6]

CAT#: CF503931

Carrier-free (BSA/glycerol-free) TUBAL3 mouse monoclonal antibody, clone OTI1G6 (formerly 1G6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBAL3 (RC216194, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBAL3. Positive lysates LY411058 (100ug) and LC411058 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TUBAL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503931)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TUBAL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503931)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TUBAL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503931)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TUBAL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503931)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TUBAL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503931)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TUBAL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503931)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TUBAL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503931)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TUBAL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503931)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TUBAL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503931)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TUBAL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503931)

• Anti-TUBAL3 mouse monoclonal antibody (TA503931) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBAL3 (RC216194).

• HEK293T cells transfected with either RC216194 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TUBAL3 antibody (TA503931), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-TUBAL3 antibody (TA503931), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-TUBAL3 antibody (TA503931), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1G6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 150-446 of human TUBAL3(NP_079079) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.7 kDa
• Gene Name: Homo sapiens tubulin alpha like 3 (TUBAL3), transcript variant 1, mRNA.
• Database Link:
  NP_079079
  Entrez Gene 79861 Human
  UniProt ID A6NHL2
• Synonyms: FLJ21665; MGC119347; MGC119349

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection