ERp72 (PDIA4) Mouse Monoclonal Antibody [Clone ID: OTI2C11]

CAT#: CF503882

Carrier-free (BSA/glycerol-free) PDIA4 mouse monoclonal antibody, clone OTI2C11 (formerly 2C11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PDIA4 (Cat# RC204041, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA4(Cat# TA503882). Positive lysates LY401531 (100ug) and LC401531 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDIA4 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PDIA4-Knockout 293T cells (KO, Cat# LC841833) were separated by SDS-PAGE and immunoblotted with anti-PDIA4 monoclonal antibody TA503882, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503882)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503882)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503882)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503882)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503882)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503882)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503882)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503882)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503882)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503882)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503882)

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Specifications

Product Data

• Clone Name: OTI2C11
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Dog, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDIA4(NP_004902) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.8 kDa
• Gene Name: Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant 2, mRNA.
• Database Link:
  NP_004902
  Entrez Gene 12304 MouseEntrez Gene 116598 RatEntrez Gene 482715 DogEntrez Gene 709716 MonkeyEntrez Gene 9601 Human
  UniProt ID P13667
• Synonyms: ERp-72; ERP70; ERP72

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Vibrio cholerae infection