NUDT18 Mouse Monoclonal Antibody [Clone ID: OTI5D7]

CAT#: CF503847

Carrier-free (BSA/glycerol-free) NUDT18 mouse monoclonal antibody, clone OTI5D7 (formerly 5D7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NUDT18 (Cat# RC204376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUDT18(Cat# TA503847). Positive lysates LY403033 (100ug) and LC403033 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NUDT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503847)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NUDT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503847)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NUDT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503847)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NUDT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503847)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NUDT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503847)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NUDT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503847)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NUDT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503847)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NUDT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503847)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NUDT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503847)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NUDT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503847)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NUDT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503847)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NUDT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503847)

• Anti-NUDT18 mouse monoclonal antibody (TA503847) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUDT18 (RC204376).

• HEK293T cells transfected with either RC204376 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NUDT18 antibody (TA503847), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5D7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NUDT18(NP_079091) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.3 kDa
• Gene Name: Homo sapiens nudix hydrolase 18 (NUDT18), mRNA.
• Database Link:
  NP_079091
  Entrez Gene 79873 Human
  UniProt ID Q6ZVK8
• Synonyms: MTH3