EDG2 (LPAR1) Mouse Monoclonal Antibody [Clone ID: OTI1G6]

CAT#: CF503737

Carrier-free (BSA/glycerol-free) LPAR1 mouse monoclonal antibody, clone OTI1G6 (formerly 1G6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LPAR1 (Cat# RC206065, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPAR1(Cat# TA503737). Positive lysates LY400545 (100ug) and LC400545 (20ug) can be purchased separately from OriGene.

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  Anti-LPAR1 mouse monoclonal antibody (TA503737) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LPAR1 (RC206065).

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  HEK293T cells transfected with either RC206065 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LPAR1 antibody (TA503737), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1G6
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LPAR1(NP_001392) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.9 kDa
• Gene Name: Homo sapiens lysophosphatidic acid receptor 1 (LPAR1), transcript variant 1, mRNA.
• Database Link:
  NP_001392
  Entrez Gene 14745 MouseEntrez Gene 116744 RatEntrez Gene 1902 Human
  UniProt ID Q92633
• Background: The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Two transcript variants encoding the same protein have been identified for this gene [provided by RefSeq, Jul
• Synonyms: edg-2; EDG2; Gpcr26; LPA1; Mrec1.3; rec.1.3; vzg-1; VZG1

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Gap junction, Neuroactive ligand-receptor interaction