SEC14 like protein 2 (SEC14L2) Mouse Monoclonal Antibody [Clone ID: OTI4H2]

CAT#: CF503723

Carrier-free (BSA/glycerol-free) SEC14L2 mouse monoclonal antibody, clone OTI4H2 (formerly 4H2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SEC14L2 (Cat# RC215994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2(Cat# TA503723). Positive lysates LY415756 (100ug) and LC415756 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SEC14L2 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SEC14L2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SEC14L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503723)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SEC14L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503723)

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  Anti-SEC14L2 mouse monoclonal antibody (TA503723) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2 (RC215994).

• HEK293T cells transfected with either RC215994 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SEC14L2 antibody (TA503723), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4H2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SEC14L2(NP_036561) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46 kDa
• Gene Name: SEC14 like lipid binding 2
• Database Link:
  NP_036561
  Entrez Gene 67815 MouseEntrez Gene 477539 DogEntrez Gene 718031 MonkeyEntrez Gene 23541 Human
  UniProt ID O76054
• Background: This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]
• Synonyms: C22orf6; SPF; TAP; TAP1

Reference Data

• Protein Families: Transcription Factors