ALS2CR1 (NIF3L1) Mouse Monoclonal Antibody [Clone ID: OTI1F6]

CAT#: CF503679

Carrier-free (BSA/glycerol-free) NIF3L1 mouse monoclonal antibody, clone OTI1F6 (formerly 1F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIF3L1 (RC200827, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIF3L1. Positive lysates LY411906 (100ug) and LC411906 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NIF3L1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503679)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503679)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503679)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503679)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503679)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503679)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503679)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503679)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503679)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NIF3L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503679)

• Anti-NIF3L1 mouse monoclonal antibody (TA503679) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIF3L1 (RC200827).

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Specifications

Product Data

• Clone Name: OTI1F6
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NIF3L1(NP_068596) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.8 kDa
• Gene Name: Homo sapiens NGG1 interacting factor 3 like 1 (NIF3L1), transcript variant 2, mRNA.
• Database Link:
  NP_068596
  Entrez Gene 301431 RatEntrez Gene 60491 Human
  UniProt ID Q9GZT8
• Synonyms: ALS2CR1; CALS-7; MDS015