RbAp46 (RBBP7) Mouse Monoclonal Antibody [Clone ID: OTI5A4]

CAT#: CF503667

Carrier-free (BSA/glycerol-free) RBBP7 mouse monoclonal antibody, clone OTI5A4 (formerly 5A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RBBP7 (Cat# RC217969, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBBP7(Cat# TA503667). Positive lysates LY419038 (100ug) and LC419038 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and RBBP7-Knockout Hela cells (KO, Cat# LC810200) were separated by SDS-PAGE and immunoblotted with anti-RBBP7 monoclonal antibody TA503667. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503667)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503667)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503667)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503667)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503667)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503667)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503667)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503667)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503667)

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Specifications

Product Data

• Clone Name: OTI5A4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinnat protein of RBBP7(NP_002884) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.6 kDa
• Gene Name: RB binding protein 7, chromatin remodeling factor
• Database Link:
  NP_002884
  Entrez Gene 83712 RatEntrez Gene 245688 MouseEntrez Gene 5931 Human
  UniProt ID Q16576
• Background: This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: RbAp46

Reference Data

• Protein Families: Druggable Genome