Ribophorin I (RPN1) Mouse Monoclonal Antibody [Clone ID: OTI5B1]

CAT#: CF503655

Carrier-free (BSA/glycerol-free) RPN1 mouse monoclonal antibody, clone OTI5B1 (formerly 5B1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPN1 (RC201554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPN1. Positive lysates LY418997 (100ug) and LC418997 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-RPN1 monoclonal antibody at 1:200.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA503655)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA503655)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA503655)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA503655)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA503655)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA503655)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA503655)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA503655)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RPN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA503655)

• Anti-RPN1 mouse monoclonal antibody (TA503655) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPN1 (RC201554).

• HEK293T cells transfected with either RC201554 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RPN1 antibody (TA503655), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5B1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RPN1(NP_002941) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66.2 kDa
• Gene Name: Homo sapiens ribophorin I (RPN1), mRNA.
• Database Link:
  NP_002941
  Entrez Gene 25596 RatEntrez Gene 6184 Human
  UniProt ID P04843
• Background: This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]
• Synonyms: OST1; RBPH1

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis