Apg3 (ATG3) Mouse Monoclonal Antibody [Clone ID: OTI3H2]

CAT#: CF503346

Carrier-free (BSA/glycerol-free) ATG3 mouse monoclonal antibody, clone OTI3H2 (formerly 3H2)

Product Images

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503346)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503346)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503346)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503346)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ATG3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503346)

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  Flow cytometric Analysis of Jurkat cells, using anti-ATG3 antibody (TA503346), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Hela cells, using anti-ATG3 antibody (TA503346), (Red), compared to a nonspecific negative control antibody, (Blue).

• HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG3 (RC203453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG3 (TA503346). Positive lysates LY411559 (100ug) and LC411559 (20ug) can be purchased separately from OriGene.

• Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATG3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• Anti-ATG3 mouse monoclonal antibody (TA503346) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATG3 (RC203453).

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Specifications

Product Data

• Clone Name: OTI3H2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ATG3(NP_071933) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.7 kDa
• Gene Name: autophagy related 3
• Database Link:
  NP_071933
  Entrez Gene 67841 MouseEntrez Gene 171415 RatEntrez Gene 478564 DogEntrez Gene 708305 MonkeyEntrez Gene 64422 Human
  UniProt ID Q9NT62
• Background: Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]). [supplied by OMIM, Mar 2008]
• Synonyms: APG3; APG3-LIKE; APG3L; PC3-96

Reference Data

• Protein Pathways: Regulation of autophagy