COASY Mouse Monoclonal Antibody [Clone ID: OTI3B3]

CAT#: CF503344

Carrier-free (BSA/glycerol-free) COASY mouse monoclonal antibody, clone OTI3B3 (formerly 3B3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY COASY (Cat# RC220733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COASY(Cat# TA503344). Positive lysates LY403068 (100ug) and LC403068 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503344)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503344)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503344)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503344)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503344)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503344)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-COASY mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503344)

• Anti-COASY mouse monoclonal antibody (TA503344) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY COASY (RC220733).

• HEK293T cells transfected with either RC220733 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-COASY antibody (TA503344), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3B3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human COASY(NP_079509) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62.1 kDa
• Gene Name: Homo sapiens Coenzyme A synthase (COASY), transcript variant 1, mRNA.
• Database Link:
  NP_079509
  Entrez Gene 71743 MouseEntrez Gene 287711 RatEntrez Gene 80347 Human
  UniProt ID Q13057
• Background: Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]). [supplied by OMIM]
• Synonyms: DPCK; NBIA6; NBP; pOV-2; PPAT; UKR1

Reference Data

• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis