Tropomodulin 1 (TMOD1) Mouse Monoclonal Antibody [Clone ID: OTI2C2]

CAT#: CF503170

Carrier-free (BSA/glycerol-free) TMOD1 mouse monoclonal antibody, clone OTI2C2 (formerly 2C2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TMOD1 (Cat# RC201134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMOD1(Cat# TA503170). Positive lysates LY418797 (100ug) and LC418797 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TMOD1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TMOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503170)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TMOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503170)

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  Anti-TMOD1 mouse monoclonal antibody (TA503170) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TMOD1 (RC201134).

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Specifications

Product Data

• Clone Name: OTI2C2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TMOD1(NP_003266) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.4 kDa
• Gene Name: Homo sapiens tropomodulin 1 (TMOD1), transcript variant 1, mRNA.
• Database Link:
  NP_003266
  Entrez Gene 21916 MouseEntrez Gene 25566 RatEntrez Gene 715838 MonkeyEntrez Gene 7111 Human
  UniProt ID P28289
• Background: This gene encodes a member of the tropomodulin family. The encoded protein is an actin-capping protein that regulates tropomyosin by binding to its N-terminus, inhibiting depolymerization and elongation of the pointed end of actin filaments and thereby influencing the structure of the erythrocyte membrane skeleton. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
• Synonyms: D9S57E; ETMOD; TMOD