SM22 alpha (TAGLN) Mouse Monoclonal Antibody [Clone ID: OTI8C8]

CAT#: CF503092

Carrier-free (BSA/glycerol-free) TAGLN mouse monoclonal antibody, clone OTI8C8 (formerly 8C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TAGLN (Cat# RC215789, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAGLN (Cat# TA503092). Positive lysates LY401104 (100ug) and LC401104 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TAGLN monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TAGLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503092)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TAGLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503092)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TAGLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503092)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TAGLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503092)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TAGLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503092)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TAGLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503092)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TAGLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503092)

• Immunohistochemical staining of paraffin-embedded human breast cancer tissue using anti-TAGLN mouse monoclonal antibody. (Heat-induced epitope retrieval, TA503092). Data courtesy of a collaborative pathologist.

• Anti-TAGLN mouse monoclonal antibody (TA503092) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TAGLN (RC215789).

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Specifications

Product Data

• Clone Name: OTI8C8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human reocombinant protein of human TAGLN(NP_003177) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.4 kDa
• Gene Name: Homo sapiens transgelin (TAGLN), transcript variant 2, mRNA.
• Database Link:
  NP_003177
  Entrez Gene 21345 MouseEntrez Gene 25123 RatEntrez Gene 6876 Human
  UniProt ID Q01995
• Background: The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
• Synonyms: SM22; SMCC; TAGLN1; WS3-10