TOMM34 Mouse Monoclonal Antibody [Clone ID: OTI2A9]

CAT#: CF503090

Carrier-free (BSA/glycerol-free) TOMM34 mouse monoclonal antibody, clone OTI2A9 (formerly 2A9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TOMM34 (Cat# RC201083, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOMM34(Cat# TA503090). Positive lysates LY416405 (100ug) and LC416405 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TOMM34 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TOMM34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503090)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TOMM34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503090)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TOMM34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503090)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TOMM34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503090)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TOMM34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503090)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TOMM34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503090)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TOMM34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503090)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TOMM34 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503090)

• Anti-TOMM34 mouse monoclonal antibody (TA503090) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TOMM34 (RC201083).

• Immunofluorescent staining of HeLa cells using anti-TOMM34 mouse monoclonal antibody (TA503090).

• HEK293T cells transfected with either RC201083 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TOMM34 antibody (TA503090), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2A9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:50~100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TOMM34(NP_006800) produced in HEK293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.4 kDa
• Gene Name: Homo sapiens translocase of outer mitochondrial membrane 34 (TOMM34), mRNA; nuclear gene for mitochondrial product.
• Database Link:
  NP_006800
  Entrez Gene 10953 Human
  UniProt ID Q15785
• Background: The protein encoded by this gene is involved in the import of precursor proteins into mitochondria. The encoded protein has a chaperone-like activity, binding the mature portion of unfolded proteins and aiding their import into mitochondria. This protein, which is found in the cytoplasm and sometimes associated with the outer mitochondrial membrane, has a weak ATPase activity and contains 6 TPR repeats. [provided by RefSeq]
• Synonyms: HTOM34P; TOM34; URCC3

Reference Data

• Protein Families: Druggable Genome