ILVBL Mouse Monoclonal Antibody [Clone ID: OTI8B12]

CAT#: CF503079

Carrier-free (BSA/glycerol-free) ILVBL mouse monoclonal antibody, clone OTI8B12 (formerly 8B12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ILVBL (RC203987, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ILVBL. Positive lysates LY416389 (100ug) and LC416389 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ILVBL monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503079)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503079)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503079)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503079)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503079)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503079)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503079)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503079)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503079)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503079)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ILVBL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503079)

• Anti-ILVBL mouse monoclonal antibody (TA503079) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ILVBL (RC203987).

• Flow cytometric Analysis of Hela cells, using anti-ILVBL antibody (TA503079), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-ILVBL antibody (TA503079), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI8B12
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ILVBL(NP_006835) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67.7 kDa
• Gene Name: Homo sapiens ilvB acetolactate synthase like (ILVBL), mRNA.
• Database Link:
  NP_006835
  Entrez Gene 362843 RatEntrez Gene 100856083 DogEntrez Gene 718086 MonkeyEntrez Gene 10994 Human
  UniProt ID A1L0T0
• Background: The protein encoded by this gene shares similarity with several thiamine pyrophosphate-binding proteins identified in bacteria, yeast, and plants. The highest degree of similarity is found with bacterial acetolactate synthases (AHAS), which are enzymes that catalyze the first step in branched-chain amino acid biosynthesis. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: 209L8; AHAS; ILV2H

Reference Data

• Protein Families: Transmembrane