OSGEP Mouse Monoclonal Antibody [Clone ID: OTI9E3]

CAT#: CF503002

Carrier-free (BSA/glycerol-free) OSGEP mouse monoclonal antibody, clone OTI9E3 (formerly 9E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY OSGEP (Cat# RC207089, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSGEP(Cat# TA503002). Positive lysates LY402616 (100ug) and LC402616 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OSGEP monoclonal antibody.

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-OSGEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503002)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-OSGEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503002)

• 

  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-OSGEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503002)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-OSGEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503002)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-OSGEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503002)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-OSGEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503002)

• Flow cytometric Analysis of Hela cells, using anti-OSGEP antibody (TA503002), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-OSGEP antibody (TA503002), (Red), compared to a nonspecific negative control antibody, (Blue).

Show all 10 images

Specifications

Product Data

• Clone Name: OTI9E3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Dog, Rat, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-335 of human OSGEP(NP_060277) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.2 kDa
• Gene Name: O-sialoglycoprotein endopeptidase
• Database Link:
  NP_060277
  Entrez Gene 66246 MouseEntrez Gene 290028 RatEntrez Gene 100687555 DogEntrez Gene 55644 Human
  UniProt ID Q9NPF4
• Synonyms: GCPL1; KAE1; OSGEP1; PRSMG1

Reference Data

• Protein Families: Druggable Genome, Protease