SNAP25 Mouse Monoclonal Antibody [Clone ID: OTI4G5]

CAT#: CF502963

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Carrier-free (BSA/glycerol-free) SNAP25 mouse monoclonal antibody, clone OTI4G5 (formerly 4G5)



  View other "OTI4G5" antibodies (4)

USD 545.00


Availability*
In Stock

Size
    • 100 ug


Specifications

Product Data
Clone Name OTI4G5
Applications FC, IF, IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity Human, Mouse, Rat
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Full length human recombinant protein of human SNAP25 (NP_003072) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 23.2 kDa
Gene Name Homo sapiens synaptosome associated protein 25 (SNAP25), transcript variant 1, mRNA.
Background Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]
Synonyms bA416N4.2; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25
Reference Data
Protein Families Druggable Genome
Protein Pathways SNARE interactions in vesicular transport

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