CRY2 Mouse Monoclonal Antibody [Clone ID: OTI1H5]

CAT#: CF502905

Carrier-free (BSA/glycerol-free) CRY2 mouse monoclonal antibody, clone OTI1H5 (formerly 1H5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CRY2 (Cat# RC207337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRY2(Cat# TA502905). Positive lysates LY432326 (100ug) and LC432326 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-CRY2 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502905)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502905)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502905)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502905)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502905)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502905)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CRY2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502905)

• Anti-CRY2 mouse monoclonal antibody (TA502905) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRY2 (RC207337 ).

• Immunofluorescent staining of HeLa cells using anti-CRY2 mouse monoclonal antibody (TA502905).

• Flow cytometric Analysis of Hela cells, using anti-CRY2 antibody (TA502905), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-CRY2 antibody (TA502905), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1H5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CRY2 (NP_0066940) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66.8 kDa
• Gene Name: Homo sapiens cryptochrome circadian regulator 2 (CRY2), transcript variant 1, mRNA.
• Database Link:
  NP_066940
  Entrez Gene 12953 MouseEntrez Gene 170917 RatEntrez Gene 1408 Human
  UniProt ID Q49AN0
• Synonyms: HCRY2; PHLL2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Circadian rhythm - mammal