PNMT Mouse Monoclonal Antibody [Clone ID: OTI1D2]

SKU CF502822

Carrier-free (BSA/glycerol-free) PNMT mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)

Product Data

• Clone Name: OTI1D2
• Application: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivity: Dog, Human, Monkey, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PNMT(NP_002677) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 30.7 kDa
• Gene Name: phenylethanolamine N-methyltransferase
• Database Link:
  NP_002677
  P11086
• Background: The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. [provided by RefSeq]
• Synonyms: PENT; PNMTase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tyrosine metabolism