BCAR1 Mouse Monoclonal Antibody [Clone ID: OTI1B1]

CAT#: CF502663

Carrier-free (BSA/glycerol-free) BCAR1 mouse monoclonal antibody, clone OTI1B1 (formerly 1B1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAR1 (RC209133, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAR1. Positive lysates LY415204 (100ug) and LC415204 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCAR1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BCAR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502663)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BCAR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502663)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BCAR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502663)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-BCAR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502663)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BCAR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502663)

• Anti-BCAR1 mouse monoclonal antibody (TA502663) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCAR1 (RC209133).

• HEK293T cells transfected with either RC209133 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BCAR1 antibody (TA502663), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-BCAR1 antibody (TA502663), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-BCAR1 antibody (TA502663), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1B1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BCAR1 (NP_055382) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 93.2 kDa
• Gene Name: Homo sapiens BCAR1 scaffold protein, Cas family member (BCAR1), transcript variant 6, mRNA.
• Database Link:
  NP_055382
  Entrez Gene 12927 MouseEntrez Gene 25414 RatEntrez Gene 479648 DogEntrez Gene 713965 MonkeyEntrez Gene 9564 Human
  UniProt ID P56945
• Background: BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]). [supplied by OMIM]
• Synonyms: CAS; CAS1; CASS1; CRKAS; P130Cas

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton