HDHD1A (PUDP) Mouse Monoclonal Antibody [Clone ID: OTI7A2]

CAT#: CF502555

Carrier-free (BSA/glycerol-free) HDHD1 mouse monoclonal antibody, clone OTI7A2 (formerly 7A2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HDHD1 (Cat# RC204419, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDHD1(Cat# TA502555). Positive lysates LY415984 (100ug) and LC415984 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HDHD1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HDHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502555)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HDHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502555)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HDHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502555)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HDHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502555)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HDHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502555)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HDHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502555)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HDHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502555)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HDHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502555)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HDHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502555)

• Flow cytometric Analysis of Hela cells, using anti-HDHD1 antibody (TA502555), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-HDHD1 antibody (TA502555), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI7A2
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HDHD1(NP_036212) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.5 kDa
• Gene Name: Homo sapiens pseudouridine 5'-phosphatase (PUDP), transcript variant 2, mRNA.
• Database Link:
  NP_036212
  Entrez Gene 67365 MouseEntrez Gene 291585 RatEntrez Gene 610678 DogEntrez Gene 707930 MonkeyEntrez Gene 8226 Human
  UniProt ID Q08623
• Background: This gene encodes a member of the haloacid dehalogenase-like (HAD) hydrolase superfamily. The encoded protein has no known biological function. This gene has a pseudogene on chromosome 1. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
• Synonyms: DXF68S1E; FAM16AX; GS1; HDHD1A