CHCHD5 Mouse Monoclonal Antibody [Clone ID: OTI3H3]

CAT#: CF502399

Carrier-free (BSA/glycerol-free) CHCHD5 mouse monoclonal antibody, clone OTI3H3 (formerly 3H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD5 (RC202606, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHCHD5. Positive lysates LY410209 (100ug) and LC410209 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CHCHD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502399)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CHCHD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502399)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CHCHD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502399)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CHCHD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502399)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CHCHD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502399)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CHCHD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502399)

• Anti-CHCHD5 mouse monoclonal antibody (TA502399) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHCHD5 (RC202606).

• HEK293T cells transfected with either RC202606 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHCHD5 antibody (TA502399), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3H3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-110 of human CHCHD5(NP_115685) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.2 kDa
• Gene Name: coiled-coil-helix-coiled-coil-helix domain containing 5
• Database Link:
  NP_115685
  Entrez Gene 84269 Human
  UniProt ID Q9BSY4
• Synonyms: C2orf9; MIC14