NUDT6 Mouse Monoclonal Antibody [Clone ID: OTI9D12]

CAT#: CF502343

Carrier-free (BSA/glycerol-free) NUDT6 mouse monoclonal antibody, clone OTI9D12 (formerly 9D12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NUDT6 (Cat# RC203470, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUDT6(Cat# TA502343). Positive lysates LY402086 (100ug) and LC402086 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NUDT6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502343)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NUDT6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502343)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NUDT6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502343)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NUDT6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502343)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NUDT6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502343)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-NUDT6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502343)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NUDT6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502343)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NUDT6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502343)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NUDT6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502343)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NUDT6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502343)

• Anti-NUDT6 mouse monoclonal antibody (TA502343) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUDT6 (RC203470).

• HEK293T cells transfected with either RC203470 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NUDT6 antibody (TA502343), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI9D12
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NUDT6 (NP_009014) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.5 kDa
• Gene Name: Homo sapiens nudix hydrolase 6 (NUDT6), transcript variant 1, mRNA.
• Database Link:
  NP_009014
  Entrez Gene 11162 Human
  UniProt ID P53370
• Background: FGF2 (MIM 134920) is a highly conserved, multifunctional heparin-binding growth factor involved in neuroectoderm development, angiogenesis, and wound healing. Elevated levels of FGF2 are associated with proliferation of smooth muscle in atherosclerosis and with proliferation of tumors. The FGF2 antisense gene, NUDT6, may regulate FGF2 expression. [supplied by OMIM]
• Synonyms: ASFGF2; FGF-AS; FGF2AS; GFG-1; GFG1