ATP6V1F Mouse Monoclonal Antibody [Clone ID: OTI1B8]

SKU
CF502279
Carrier-free (BSA/glycerol-free) ATP6V1F mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)
  • TrueMAB

    Antibodies made against full-length proteins as antigens.

    Click here to learn more.

$600.00
3 Days*
Specifications
Product Data
Clone Name OTI1B8
Application FC, IF, IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
Reactivity Human, Mouse, Rat
Antibody Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Full length human recombinant protein of human ATP6V1F (NP_004222) produced in E.coli.
Buffer Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Ambient
Predicted Protein Size 13.2 kDa
Gene Name ATPase H+ transporting V1 subunit F
Database Link
Background This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq]
Synonyms ATP6S14; VATF; Vma7
Reference Data
Protein Pathways Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
Write Your Own Review
You're reviewing:ATP6V1F Mouse Monoclonal Antibody [Clone ID: OTI1B8]
Your Rating
SKU Description Size Price
TA502279 ATP6V1F mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 100 ul
$447.00
TA502279AM ATP6V1F mouse monoclonal antibody, clone OTI1B8 (formerly 1B8), Biotinylated 100 ul
$509.00
TA502279BM ATP6V1F mouse monoclonal antibody, clone OTI1B8 (formerly 1B8), HRP conjugated 100 ul
$509.00
TA502279S ATP6V1F mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) 30 ul
$200.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.