ATP6V1F Mouse Monoclonal Antibody [Clone ID: OTI1B8]
SKU
CF502279
Carrier-free (BSA/glycerol-free) ATP6V1F mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)
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TrueMAB™
Antibodies made against full-length proteins as antigens.
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Product Data | |
Clone Name | OTI1B8 |
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Application | FC, IF, IHC, WB |
Recommended Dilution | WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100 |
Reactivity | Human, Mouse, Rat |
Antibody Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Full length human recombinant protein of human ATP6V1F (NP_004222) produced in E.coli. |
Buffer | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Shipping | Ambient |
Predicted Protein Size | 13.2 kDa |
Gene Name | ATPase H+ transporting V1 subunit F |
Database Link | |
Background | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq] |
Synonyms | ATP6S14; VATF; Vma7 |
Reference Data | |
Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
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Product Manuals |
FAQs |
SDS |
Antibody Resources |
SKU | Description | Size | Price | |
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TA502279 | ATP6V1F mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) | 100 ul |
$447.00
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TA502279AM | ATP6V1F mouse monoclonal antibody, clone OTI1B8 (formerly 1B8), Biotinylated | 100 ul |
$509.00
|
|
TA502279BM | ATP6V1F mouse monoclonal antibody, clone OTI1B8 (formerly 1B8), HRP conjugated | 100 ul |
$509.00
|
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TA502279S | ATP6V1F mouse monoclonal antibody, clone OTI1B8 (formerly 1B8) | 30 ul |
$200.00
|
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.