NMNAT1 Mouse Monoclonal Antibody [Clone ID: OTI1G4]

CAT#: CF502220

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Carrier-free (BSA/glycerol-free) NMNAT1 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)



  View other "OTI1G4" antibodies (4)

USD 545.00


Availability*
In Stock

Size
    • 100 ug


Specifications

Product Data
Clone Name OTI1G4
Applications FC, IHC, WB
Recommended Dilution WB 1:500~2000, IHC 1:150, FLOW 1:100
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human NMNAT1 (NP_073624) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 31.8 kDa
Gene Name Homo sapiens nicotinamide nucleotide adenylyltransferase 1 (NMNAT1), transcript variant 1, mRNA.
Background The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT (EC 2.7.7.1) is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD (Zhang et al., 2003 [PubMed 12574164]). [supplied by OMIM]
Synonyms LCA9; NMNAT; PNAT1; SHILCA
Reference Data
Protein Pathways Metabolic pathways, Nicotinate and nicotinamide metabolism

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