KEAP1 Mouse Monoclonal Antibody [Clone ID: OTI1B4]

CAT#: CF502059

Carrier-free (BSA/glycerol-free) KEAP1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY KEAP1 (Cat# RC202189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (Cat# TA502059)(1:1000).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-KEAP1 monoclonal antibody (1:200).

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  Figure from citation: Western Blot of Keap1 protein level by using anti-Keap1 antibody in human BEAS2B cells. View Citation

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and KEAP1-Knockout Hela cells (KO, Cat# LC810295) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 monoclonal antibody TA502059. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).

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  Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY KEAP1 (RC202189) using anti-KEAP1 antibody (TA502059/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).

• HEK293T cells transfected with either RC202189 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody (TA502059), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-KEAP1 antibody (TA502059), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-KEAP1 antibody (TA502059), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1B4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinnt protein of human KEAP1(NP_987096) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 69.5 kDa
• Gene Name: kelch like ECH associated protein 1
• Database Link:
  NP_987096
  Entrez Gene 50868 MouseEntrez Gene 117519 RatEntrez Gene 9817 Human
  UniProt ID Q14145
• Background: This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq]
• Synonyms: INrf2; KLHL19

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Ubiquitin mediated proteolysis