PYCR3 Mouse Monoclonal Antibody [Clone ID: OTI8H6]

CAT#: CF502036

Carrier-free (BSA/glycerol-free) PYCRL mouse monoclonal antibody, clone OTI8H6 (formerly 8H6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PYCRL (Cat# RC229141, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCRL(Cat# TA502036). Positive lysates LY411510 (100ug) and LC411510 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PYCRL monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

• Anti-PYCRL mouse monoclonal antibody (TA502036) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCRL (RC203382).

• HEK293T cells transfected with either RC203382 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCRL antibody (TA502036), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-PYCRL antibody (TA502036), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI8H6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PYCRL (NP_075566) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.5 kDa
• Gene Name: Homo sapiens pyrroline-5-carboxylate reductase 3 (PYCR3), transcript variant 1, mRNA.
• Database Link:
  NP_075566
  Entrez Gene 65263 Human
  UniProt ID Q53H96
• Synonyms: PYCR3

Reference Data

• Protein Pathways: Arginine and proline metabolism, Metabolic pathways