XLF (NHEJ1) Mouse Monoclonal Antibody [Clone ID: OTI3D6]

CAT#: CF502007

Carrier-free (BSA/glycerol-free) NHEJ1 mouse monoclonal antibody, clone OTI3D6 (formerly 3D6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NHEJ1 (Cat# RC203393, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1(Cat# TA502007). Positive lysates LY403031 (100ug) and LC403031 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NHEJ1 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and NHEJ1-Knockout HeLa cells (KO, Cat# LC831412) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1 monoclonal antibody TA502007 (1:200). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502007)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502007)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502007)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502007)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502007)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502007)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502007)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502007)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502007)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502007)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502007)

• Anti-NHEJ1 mouse monoclonal antibody (TA502007) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NHEJ1 (RC203393).

• Flow cytometric Analysis of Hela cells, using anti-NHEJ1 antibody (TA502007), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI3D6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NHEJ1 (NP_079058) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.2 kDa
• Gene Name: non-homologous end joining factor 1
• Database Link:
  NP_079058
  Entrez Gene 701542 MonkeyEntrez Gene 79840 Human
  UniProt ID Q9H9Q4
• Background: Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq]
• Synonyms: XLF

Reference Data

• Protein Pathways: Non-homologous end-joining