ZNRD2 Mouse Monoclonal Antibody [Clone ID: OTI2F5]

CAT#: CF501915

Carrier-free (BSA/glycerol-free) SSSCA1 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SSSCA1 (Cat# RC201946, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSSCA1(Cat# TA501915). Positive lysates LY416665 (100ug) and LC416665 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSSCA1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) at 1:200 dilution.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SSSCA1 monoclonal antibody (1:200).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and SSSCA1-Knockout 293T cells (KO, Cat# LC842424 ) were separated by SDS-PAGE and immunoblotted with anti-SSSCA1 monoclonal antibody TA501915 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SSSCA1 mouse monoclonal antibody. (TA501915)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSSCA1 mouse monoclonal antibody. (TA501915)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SSSCA1 mouse monoclonal antibody. (TA501915)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SSSCA1 mouse monoclonal antibody. (TA501915)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SSSCA1 mouse monoclonal antibody. (TA501915)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SSSCA1 mouse monoclonal antibody. (TA501915)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SSSCA1 mouse monoclonal antibody. (TA501915)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SSSCA1 mouse monoclonal antibody. (TA501915)

• Anti-SSSCA1 mouse monoclonal antibody (TA501915) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSSCA1 (RC201946).

• HEK293T cells transfected with either RC201946 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SSSCA1 antibody (TA501915), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-SSSCA1 antibody (TA501915), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-SSSCA1 antibody (TA501915), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI2F5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100, IHC: 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SSSCA1 (NP_006387) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.3 kDa
• Gene Name: Homo sapiens zinc ribbon domain containing 2 (ZNRD2), transcript variant 1, mRNA.
• Database Link:
  NP_006387
  Entrez Gene 10534 Human
  UniProt ID O60232
• Background: This antigen is recognized by a subset of anti-centromere antibodies from patients with scleroderma and/or Sjogren's syndrome. Subcellular localization has not yet been established. [provided by RefSeq]
• Synonyms: p27