Glutathione S Transferase theta 2 (GSTT2) Mouse Monoclonal Antibody [Clone ID: OTI2E3]

CAT#: CF501829

Carrier-free (BSA/glycerol-free) GSTT2 mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSTT2 (RC200040, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSTT2. Positive lysates LY424485 (100ug) and LC424485 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GSTT2 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GSTT2 mouse monoclonal antibody at 1:150 (TA501829)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GSTT2 mouse monoclonal antibody at 1:150 (TA501829)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GSTT2 mouse monoclonal antibody at 1:150 (TA501829)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GSTT2 mouse monoclonal antibody at 1:150 (TA501829)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GSTT2 mouse monoclonal antibody at 1:150 (TA501829)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GSTT2 mouse monoclonal antibody at 1:150 (TA501829)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GSTT2 mouse monoclonal antibody at 1:150 (TA501829)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GSTT2 mouse monoclonal antibody at 1:150 (TA501829)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GSTT2 mouse monoclonal antibody at 1:150 (TA501829)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-GSTT2 mouse monoclonal antibody at 1:150 (TA501829)

• Flow cytometric Analysis of Hela cells, using anti-GSTT2 antibody (TA501829), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-GSTT2 antibody (TA501829), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI2E3
• Applications: FC, IHC, WB
• Recommended Dilution: WB: 1:500-1:2000, FLOW: 1:100, IHC: 1:50-1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GSTT2 (NP_000845) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.3 kDa
• Gene Name: Homo sapiens glutathione S-transferase theta 2 (gene/pseudogene) (GSTT2), transcript variant 1, coding, mRNA.
• Database Link:
  NP_000845
  Entrez Gene 2953 Human
  UniProt ID P0CG29
• Background: Glutathione S-transferase (GSTs) theta 2 (GSTT2) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: Alpha, Mu, Pi, Theta, and Zeta. The theta class members GSTT1 and GSTT2 share 55% amino acid sequence identity and both are thought to have an important role in human carcinogenesis. The theta genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq]
• Synonyms: GSTT2B

Reference Data

• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450