EPM2AIP1 Mouse Monoclonal Antibody [Clone ID: OTI2G3]

CAT#: CF501797

Carrier-free (BSA/glycerol-free) EPM2AIP1 mouse monoclonal antibody, clone OTI2G3 (formerly 2G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EPM2AIP1 (Cat# RC209239, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPM2AIP1(Cat# TA501797). Positive lysates LY415014 (100ug) and LC415014 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of Jurkat cells, using anti-EPM2AIP1 antibody (TA501797), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  HEK293T cells transfected with either RC209239 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EPM2AIP1 antibody (TA501797), and then analyzed by flow cytometry.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

• Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-EPM2AIP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501797)

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Specifications

Product Data

• Clone Name: OTI2G3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EPM2AIP1 (NP_055620) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.2 kDa
• Gene Name: Homo sapiens EPM2A interacting protein 1 (EPM2AIP1), mRNA.
• Database Link:
  NP_055620
  Entrez Gene 77781 MouseEntrez Gene 316021 RatEntrez Gene 9852 Human
  UniProt ID Q7L775
• Background: The EPM2A gene, which encodes laforin, is mutated in an autosomal recessive form of adolescent progressive myoclonus epilepsy. The protein encoded by this gene binds to laforin, but its function is not known. This gene is intronless. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: FLJ11207; KIAA0766