CBWD1 Mouse Monoclonal Antibody [Clone ID: OTI3F9]

CAT#: CF501612

Carrier-free (BSA/glycerol-free) CBWD1 mouse monoclonal antibody, clone OTI3F9 (formerly 3F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CBWD1 (Cat# RC222790, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBWD1(Cat# TA501612). Positive lysates LY402692 (100ug) and LC402692 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CBWD1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CBWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501612)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CBWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501612)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CBWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501612)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CBWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501612)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CBWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501612)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CBWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501612)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CBWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501612)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CBWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501612)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CBWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501612)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CBWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501612)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CBWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501612)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CBWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501612)

• Anti-CBWD1 mouse monoclonal antibody (TA501612) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CBWD1 (RC222790).

• HEK293T cells transfected with either RC222790 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CBWD1 antibody (TA501612), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-CBWD1 antibody (TA501612), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI3F9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CBWD1 (NP_060961) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.9 kDa
• Gene Name: Homo sapiens COBW domain containing 1 (CBWD1), transcript variant 1, mRNA.
• Database Link:
  NP_060961
  Entrez Gene 55871 Human
  UniProt ID Q9BRT8
• Synonyms: COBP