Ephrin A2 (EFNA2) Mouse Monoclonal Antibody [Clone ID: OTI3E3]

CAT#: CF501461

Carrier-free (BSA/glycerol-free) EFNA2 mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)

Product Images

Anti-EFNA2 mouse monoclonal antibody (TA501461) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EFNA2 (RC213728).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EFNA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501461)

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  Flow cytometric Analysis of Jurkat cells, using anti-EFNA2 antibody (TA501461), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human EFNA2 plasmid (RC213728, hEFNA2), mouse EFNA2 plasmid (MR202249, mEFNA2) using anti-EFNA2 antibody TA501461(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Specifications

Product Data

• Clone Name: OTI3E3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EFNA2 (NP_001396) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.3 kDa
• Gene Name: ephrin A2
• Database Link:
  NP_001396
  Entrez Gene 1943 Human
  UniProt ID O43921
• Background: This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq]
• Synonyms: ELF-1; EPLG6; HEK7-L; LERK-6; LERK6

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance