CD5 Mouse Monoclonal Antibody [Clone ID: OTI7A7]

CAT#: CF501335

Carrier-free (BSA/glycerol-free) CD5 mouse monoclonal antibody, clone OTI7A7 (formerly 7A7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5. (1:5. Positive lysates LY415443 (100ug) and LC415443 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 1 cell line lysate by using anti-CD5 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501335, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501335, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501335, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501335, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA501335) (1:500)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA501335) (1:500)

• Anti-CD5 mouse monoclonal antibody (TA501335) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD5 (RC206494).

• HEK293T cells transfected with either RC206494 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD5 antibody (TA501335), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody (TA501335), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI7A7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.4 kDa
• Gene Name: Homo sapiens CD5 molecule (CD5), transcript variant 1, mRNA.
• Database Link:
  NP_055022
  Entrez Gene 921 Human
  UniProt ID P06127
• Synonyms: LEU1; T1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage