elF2 alpha (EIF2S1) Mouse Monoclonal Antibody [Clone ID: OTI3H4]

CAT#: CF501313

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Carrier-free (BSA/glycerol-free) EIF2S1 mouse monoclonal antibody, clone OTI3H4 (formerly 3H4)

Formulation: Standard Carrier-Free



USD 600.00

In Stock*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI3H4
Applications FC, IF, IHC, WB
Recommended Dilution WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Reactivities Human, Dog, Rat, Monkey, Mouse
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 35.9 kDa
Gene Name Homo sapiens eukaryotic translation initiation factor 2 subunit alpha (EIF2S1), mRNA.
Background The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]). [supplied by OMIM]
Synonyms EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.