Ornithine Decarboxylase (ODC1) Mouse Monoclonal Antibody [Clone ID: OTI1G6]

CAT#: CF501286

Carrier-free (BSA/glycerol-free) ODC1 mouse monoclonal antibody, clone OTI1G6 (formerly 1G6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ODC1 (Cat# RC206858, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ODC1(Cat# TA501286). Positive lysates LY400909 (100ug) and LC400909 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ODC1 monoclonal antibody.

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  Anti-ODC1 mouse monoclonal antibody (TA501286) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ODC1 (RC206858).

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  HEK293T cells transfected with either RC206858 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ODC1 antibody (TA501286), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-ODC1 antibody (TA501286), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1G6
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ODC1 (NP_002530) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.0 kDa
• Gene Name: ornithine decarboxylase 1
• Database Link:
  NP_002530
  Entrez Gene 18263 MouseEntrez Gene 24609 RatEntrez Gene 695625 MonkeyEntrez Gene 4953 Human
  UniProt ID P11926
• Background: This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. [provided by RefSeq]
• Synonyms: BABS; NEDBA; NEDBIA; ODC

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Glutathione metabolism, Metabolic pathways