TACC3 Mouse Monoclonal Antibody [Clone ID: OTI6F1]

CAT#: CF501261

Carrier-free (BSA/glycerol-free) TACC3 mouse monoclonal antibody, clone OTI6F1 (formerly 6F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TACC3 (RC210754, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TACC3. Positive lysates LY416710 (100ug) and LC416710 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TACC3 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and TACC3-Knockout 293T cells (KO, Cat# LC812337) were separated by SDS-PAGE and immunoblotted with anti-TACC3 monoclonal antibody TA501261, (1:200). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501261, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501261, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501261, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501261, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501261, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501261, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TACC3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501261, Dilution 1:50)

• Anti-TACC3 mouse monoclonal antibody (TA501261) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TACC3 (RC210754 ).

• HEK293T cells transfected with either RC210754 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TACC3 antibody (TA501261), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI6F1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TACC3(NP_006333) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 90.2 kDa
• Gene Name: Homo sapiens transforming acidic coiled-coil containing protein 3 (TACC3), mRNA.
• Database Link:
  NP_006333
  Entrez Gene 712898 MonkeyEntrez Gene 10460 Human
  UniProt ID Q9Y6A5
• Background: The function of this gene has not yet been determined; however, it is speculated that it may be involved in cell growth and differentiation. Expression of this gene is up-regulated in some cancer cell lines, and in embryonic day 15 in mice.
• Synonyms: ERIC-1; ERIC1