HIF1 beta (ARNT) Mouse Monoclonal Antibody [Clone ID: OTI1A1]
CAT#: CF501147
Carrier-free (BSA/glycerol-free) ARNT mouse monoclonal antibody, clone OTI1A1 (formerly 1A1)
Formulation: Standard
Specifications
Product Data | |
Clone Name | OTI1A1 |
Applications | FC, IF, IHC, WB |
Recommended Dilution | WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100 |
Reactivities | Human, Mouse, Rat |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Full length human recombinant protein of human ARNT (NP_001659) produced in HEK293T cell. |
Formulation | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Predicted Protein Size | 86.5 kDa |
Gene Name | aryl hydrocarbon receptor nuclear translocator |
Database Link | |
Background | The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1). A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Three alternatively spliced variants encoding different isoforms have been described for this gene. |
Synonyms | bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO |
Reference Data | |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Pathways in cancer, Renal cell carcinoma |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Antibody Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
TA501147 | ARNT (HIF1 beta) mouse monoclonal antibody, clone OTI1A1 (formerly 1A1) |
USD 447.00 |
|
TA501147AM | ARNT (HIF1 beta) mouse monoclonal antibody, clone OTI1A1 (formerly 1A1), Biotinylated |
USD 509.00 |
|
TA501147BM | ARNT (HIF1 beta) mouse monoclonal antibody, clone OTI1A1 (formerly 1A1), HRP conjugated |
USD 509.00 |
|
TA501147S | ARNT (HIF1 beta) mouse monoclonal antibody, clone OTI1A1 (formerly 1A1) |
USD 200.00 |
|
TA700042 | ARNT biotinylated mouse monoclonal detection antibody, validated for Luminex assays |
USD 494.00 |
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